Acetone And Spinach


Oct 3, 2008 isolation of chlorophyll and carotenoid pigments from spinach add 2.0 ml of acetone. rinse the mortar and pestle with another 2.0 ml of (http://www.uwlax.edu/faculty/koster/spinach.htm)


Add 2 pipet bulbs of acetone and carefully grind the spinach and acetone together with the pestle. continue grinding until the acetone becomes dark green in (http://www.upb.pitt.edu/uploadedfiles/about/sponsored_programs/science_in_motion)
Yes, acetone vapor is extremely flammable and potentially explosive. you can find reports online of explosions with spinach leaves and acetone in a blender. (http://www.madsci.org/posts/archives/2006-05/1148591631.bt.r.html)

Day of the lab – pour acetone and spinach solution through filter paper. place pigment solution in a corked bottle. follow directions listed in the lab (http://www.ncsec.org/cadre2/team24_2/plantpigments.htm)

Glycolipids of brain and spinach leaves from silicic acid columns with acetone was demonstrated. cerebrosides and sulfatides (http://www.springerlink.com/index/gh42rv3568183857.pdf)

Chl was extracted with acetone from spinach leaves and precipitated as a chl- dioxane complex by the addition of dioxane and water to the acetone extract. (http://www.jbc.org/content/271/3/1475.full)

Acetone is a good solvent for extracting these non-polar pigments from the spinach leaves. (as well as some other pigments in smaller amounts. whether (http://fog.ccsf.cc.ca.us/pherrman/documents/spinachlab208.pdf)

Add about 50ml of an 80:20 volume-to-volume mixture of petroleum ether and acetone to the spinach. grind the mixture until the solvent takes on a deep, (http://www.chem.indiana.edu/community/demos/2-2columnchromatographyofplantpigmen)

  • Materials and methods plant material spinach plants were grown as nitrogen precipitation from a chloroplast suspension by 8o% acetone aliquots (o.i ml) (http://linkinghub.elsevier.com/retrieve/pii/0005272869902278)
  • A pathway for steryl ester biosynthesis in acetone powder preparations from spinach (spinacia oleracea l.) leaves has been elucidated; free sterol and 1 (http://trophort.com/detail/162862/identification-of-an-acyl-donor-in-steryl-este)
  • Acetone powders of a 20000g pelet fraction from spinach leaves .. in spinach leaf acetone powder preparations because: (a) there (http://www.plantphysiol.org/cgi/reprint/61/3/354.pdf)
  • Spinach leaves by the method including acetone precipitation, ammo- nium sulfate fractionations and deae-cellulose column chromatography. (http://pcp.oxfordjournals.org/cgi/reprint/7/4/643.pdf)
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  • Porated into steryl esters wben incubated with spinach leaf acetone powder preparations. the optimal buffer for steryl ester biosynthesis was 2-(n- (http://www.jstor.org/stable/4265199)
  • Spinach with a pestle with about 5-10 ml of an 80:20 mixture (v:v), petroleum ether and acetone. decant the liquid into a 50 ml round bottom flask. (http://web.fccj.org/~smilczan/ten/chloro.pdf)

Bility of a spinach leaf homogenate is not sedimented by cen- trifugation for 60 min at 100000 g. an acetone powder of spinach chloroplasts contains enzymes (http://www.jlr.org/cgi/reprint/10/6/623.pdf)


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  • An acetone-ether extract of dried spring spinach has been prepared more than 200 times as concentrated as the fresh leaves, and practically free (http://www.biochemj.org/bj/021/0887/0210887.pdf)
  • Cut or tear the spinach leaves into small pieces and place them in a mortar along with 2.0 ml of acetone. grind with a pestle until the spinach (http://voh.chem.ucla.edu/vohtar/spring03/classes/14cl/pdf/14clisol.pdf)